IN VITRO ASSESSMENT OF ALOE VERA GEL EXTRACTS FOR ANTIBACTERIAL EFFICACY

DOI: https://doie.org/10.10399/JBSE.2025475049

Shakuntala Tiwari, Dr. B. Nageshwari, Dr. Masqood Ahmed Mohammad, K. Subhashini Devi, Dr. D. Sailaja

Keywords:

Aloe Vera Gel, Antibacterial, Zone of Inhibition (ZOI), Pathogens

Abstract:

This study investigates the antibacterial properties of Aloe vera gel extracts, specifically targeting common pathogenic bacteria including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas spp., Vibrio Parahaemolyticus, and Escherichia coli, as well as components of the normal flora. The Aloe vera extract antimicrobial evaluation was determined using the healthy diffusion technique. Ethanol was the solvent because it efficiently isolated the gel's bioactive compound. Ethanol containing aloe vera extract at a concentration of 20 mg/ml showed an antibacterial effect that could be measured. The extract's most significant inhibition was against the Pseudomonas spp.. In contrast, the least impact was noticed against Klebsiella pneumoniae. The size of the inhibition zones was correctly measured and compared with the ones caused by standard antibiotic discs, i.e., Gentamycin, Norfloxacin, and Streptomycin. The Aloe vera ethanol extract demonstrated greater or similar antibacterial properties than conventional antibiotics, especially with more resistant strains. This implies that Aloe vera contains highly active antimicrobial compounds that can be used as alternatives or supplements to traditional antibiotics. The results justify using Aloe vera extracts to fabricate natural antibacterial compounds, even with the rising antibiotic resistance.

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